简介：华中科技大学硕士学位论文闪蝶鳞翅微纳结构光学特性仿真与实验研究姓名曹艳波申请学位级别硕士专业机械电子工程指导教师廖广兰史铁林20090520IIABSTRACTTHECOLSOFMIRCONANOSTRUCTUREINBUTTERFLYWINGSSCALESASASPECIALBIOPHYSICALPHENOMENONEXISTINGINTHEWLDHAVEBEENPAIDMEATTENTIONBYMANYRESEARCHERSTHEFMATIONMECHANISMAPPLICATIONINVOLVESPHYSICSCHEMISTRYBIOLOGYMATERIALSCIENCEENGINEERINGOTHERFIELDSWHICHISAMULTIDISCIPLINEINTERACTIONSUBJECTHASBEETHERESEARCHFEFRONTHOTSPOTINTERNATIONALLYINTHISTHESISTHESHAPESSTRUCTURESOFFOURKINDSOFTYPICALBUTTERFLIES’SCALESEUPLOCAALGEAPAPILIOULYSSESMPHODIDIUSMPHORHETENARESTUDIEDSYSTEMATICALLYTHEDIFFERENTCONFIGURATIONSAREFOUNDTHEDETAILEDMICRONANOSHAPESSTRUCTUREOFTHEBUTTERFLYSSCALESARESTUDIEDTHEFMATIONMECHANISMOFTHESTRUCTURALCOLISREVEALEDSUCCESSFULLYTHEOPTICALMICROSCOPYSCANNINGELECTRONMICROSCOPYTRANSMISSIONELECTRONMICROSCOPYAREUSEDTOOBSERVETESTANALYZETHESURFACECROSSSECTIONMPHOLOGIESOFTHESCALESOFTHEBUTTERFLIESTHELAMELLAERIDGESOFMPHOSCALESAREFOUNDARRANGINGINANDERLYMANNERINTHEPHYSICALSTRUCTURESEACHCYCLEOFTHEMULTILAYEREDSTRUCTURECOMPOSINGOFCHITINAIRDIELECTRICINREGULARDISTRIBUTIONTHESMALLDIFFERENCESOFTHEGRATINGSLAYERSONTHESCALESMATERIALREFRACTIONRATESIZESPACINGTHICKNESSNUMBERSOFLAYERSMODEOFARRANGEMENTWILLIMPACTONTHELIGHTREFLECTIONINTERFERENCEEFFECTTWOSPECTROMETERSYSTEMSOFTHEREFLECTIONPROBETHEINTEGRATINGSPHEREARECONSTRUCTEDTOMEASURETHEREFLECTIONEFFICIENCYCOLIONPERFMANCEOFMPHOSCALESINTHEVISIBLERANGEOFLIGHTTHERELATIONBETWEENTHESHAPESSTRUCTURESOFMPHOSCALESTHEIROPTICALPROPERTIESISREVEALEDTHETESTONDISCOLATIONISDESIGNEDBYCHANGINGTHEINCIDENTANGLEFILLINGTWOKINDSOFMEDIUMOVERTHEMPHOBACKWINGSTHEDISCOLATIONISSTUDIEDFINALLYTHETHEIESSUCHASMULTILAYERTHINFILMINTERFERENCEDIFFRACTIONGRATINGAREEMPLOYEDTOEXPLAINTHEOPTICALMECHANISMOFTHESTRUCTURALCOLDISCOLATIONOFMPHOSCALESRESPECTIVELYACCDINGTODIFFRACTIONGRATINGTHEYWEUSETHERIGOUSCOUPLEDWAVEANALYSISFTHEETICALCALCULATIONOFVECTDIFFRACTIONPROBLEMSWEHAVEESTABLISHEDFOURDIFFERENTDIFFRACTIONGRATINGMODELSWITHSIMILARCONFIGURATIONBASEDONONEDIMENSIONALRIGOUSCOUPLEDWAVEANALYSISSIMULATEDTHEDIFFRACTIONEFFICIENCYAFTERGETTINGDIFFERENTREFLECTIONEFFICIENCIESWITHDIFFERENTINCIDENTANGLESDIFFERENT

简介：北京交通大学硕士学位论文基于微注射的微动并联机器人机构研究姓名高永立申请学位级别硕士专业机械设计及理论指导教师房海蓉20071201韭塞銮遵叁堂亟±堂擅论毫△垦S基△￡ABSTRACTABSFRRACTTHEBIOENGINEERINGHASDEVELOPEDRAPIDLYWITHTHEDEVELOPMENTOFTHEMICROMANIPULATORINTHERECENTYEARS．TLLISPAPERSTUDIESTHEPARALLELMICROMANIPULATOROFCELLMICROINJEOTION,FOCUSING011THEAPPLICATIONFIELDOFTHECELLMICROINJECTIONMANIPULATEANDCOMPOSEDTHECHARACTERISTICSOFTHEPARALLELMICROMANIPULATOR．111ECONTENTASFOLLOWS1．NLECONFIGURATIONOFTHELOWERDOFPARALLELMICROMANIPULATORISSTUDIEDBASEDONTHEANALYSISOFTHEMICROINJCCTIONOPERATIONREQUESTANDTHECHARACTERISTICSOFTHEPARALLELMICROMANIPULATORSTRUCTURE．1HETHEORYBASISFORTHECONFIGURATIONOFMICROMANIPULATORISESTABLISHED．2．THELIMBSTRUCTURETHATTHEMOVINGPLATFORMCANREALIZETHETHREETRANSLATIONISCOMPAREDANDANALYSED．N他3RUUPARALLELMANIPULATORISCONTRASTED．ANEW3RUUFLEXUREPARALLELMANIPULATORISDESIGNED，DISPOSEDBYPERPENDICULARITY,THROUGHTHEREPLACEMENTBYTHEFLEXUREHINGES．NE3DMODELISESTABLISHED．3．THERELATIONSHIPBETWEENTHEKINEMATICSACCURACYANDTHESTRUCTUREANDCOMPLIANCEOFSEVERALKINDSOFFLEXUREHINGESISSTUDIEDBASEDTHECHARACTERISTICSOFMICROMANIPULATOR．PAYATTENTIONTOTHECHANGESOFTHEROTATIONAXISWHENTHEFLEXUREHINGESISAFFECTEDBYTHEFORCEORTORQUE，ANDPRESENTEDTHEFLEXUREHINGESDESIGNMETHODTHATCREATETHECORRESPONDINGSTRUCTUREFLEXUREHINGESBYTHEDIFFERENTFORCE．4．11把KINEMATICSCHARACTERISTICSOFTHE3RLRUFLEXUREPARRALDMANIPULATORISANALYSED，ANDTHEFORWARDANDINVERSEKINEMATICSOFTHE3RUUMICROINJECTIONMANIPULATORISESTABLISHED．N坞MODELWASANALYSEDBYMATLABSOFTWARE．FINITEDEMENTANLYSISOFTHE3RUUCELLINJECTIONISCARRIEDOUT,ANDTHERESULTOFSIMULATIONANALYSISSHOWSTHATTHEKINEMATICSCHARACTERISFITFORTHEREQUESTOFTHESUBJECT．KEYWORDSBIOENGINEERING；,MICROINJECTION；PARRALELMANIPULATOR；FLEXUREHINGE；KINEMATICSANALYSIS．CLASSNOTHLL2

简介：大连理工大学硕士学位论文基于单片机和FPGA的CFPCR芯片系统研究姓名施兴慧申请学位级别硕士专业测试计量技术及仪器指导教师闫卫平20071220大连理工大学硕士学位论文STUDYOFCFPCRCHIPBASEDONMCUANDFPGAABSTRACTPOLYMERASECHAINREACTIONRPCRTECHNOLOGYISA1CINDOFDNAAMPLIFICATIONTECHNIQUEOUTSIDETHEBODY，WHICHHASBEENWIDELYAPPLIEDTOBIOMEDICINEANDOTHERRELATEDFIDDSATPRESENTTHEVASTMAJORITYOFPCRREACTIONISCARRIEDOUTINTHETRADITIONALCOMMERCIALPCILWHICHUSESLARGERMETALSTOCONTROLTHERMALCYCLINGTEMPERATUREBECAUSEMETALHASBIGHEATCAPACITYTHEELEVATI∞ANDDECREASESPEEDOFTEMPERATUREISSLOWERACOMPLETEPCRCYCLEUSUALLYREQUIRESLH～2HANDEV∞LONGERP甜ODOFTIMEINADDITION，CAPILLARYELECTROPHORESISANALYSISOFMICROFLUIDICDETECTIONSYSTEMHASBEENABLETOCOMPLETINASMALLERVOLUMECHIPIFTHECONTINUOUSFLOWPCRCHIPCFPCRANDCAPILLARYELCCTROPHORESISANALYSISCHIPRREINTEGRATEDTOGETHERBINCHEMICALANALYSISWILLACHIEVEINTEGRATIONANDMINIATURIZATIONTHERE触，THISPAPERDASIGNSCFPCRCHIPSYSTEMBASEDONMCUANDFPGA，WHICHMAINLYSTUDYSONPCRCHIPPRODUCTION、AMPLIFICATIONANDTEMPERATURECONTROLSYSTEMTHISPAPERFABRICATESACFPCRCHIP、ⅣITLLTHEMICROELECTRONICTECHNIQUEANDMICROMECHANICALPROCESSBYUSINGLABORATORYPRODESIGNEDCFPCRCHIPTECHNOLOGYTERRITORYINCLUDINGAMICROFLOWTHROUGHCHIPOFASSMATERIAL，ANDTHREESILICONCHIPSCONSISTOFPTMEMBRANEMICROHEATERSANDTEMPERATURESE璐ORSN圮INTELLIGENCETEMPERATURECONTROLSYSTEMISDESIGNEDBASEDONMCUANDFPGAPLATFORMWHICHSEPARATESTHETEMPERATURECONTROLALGORITHMFROMMCUBYUSINGFPGATOACHIEVETHISDESIGNNOTONLYDISPLAYSMANYADVANTAGESOFFPGA，SUCHASHIGHINTEGRATION、RELIABILITYWELL、DESIGNINGFLEXIBILITY、FASTERCOMPUTINGSPEEDAND80ONBUTALSEGREATLYIMPROVESTHETEMPERATURECONTROLPRECISIONAMONGTHEM，THEMCUMAINLYREALIZESFUNCTIONSOFADSAMPLINGCONTROL，KEYBOARDKEYSCONTROLANDTRANSMITTINGDATADIRECTIVETOFPGA；FPGAMAINLYREALIZESFUNCTIONSOFTEMPERATURECURVEFITTINGPIDCONTROLALGORITHMASWELLASPWMWAVEFORMGENERATORWITHTHECOMBINATIONOFEXTERNALHARDWARECIRCUITTHISSYSTEMCOMPLETESAMULTICHANNELTEMPERATURESIGNALSINTHEACQUISITION、CONVERSION、DISPLAYANDOTHERFUNCTIONSTHISSYSTEMEFFECTIVELYREALIZATESTHETHREETEMPERATURETHERMOSTATCONTR01BYUSINGCFPCRCHIPSYSTEMPLANTDNASAMPLEISAMPLIFIEDANDTHEPCRPRODUCTISDETECTEDBYMEANSOFAGAROSEGELELECTROPHORESISTHEEXPERIMENTALRESULTSSHOWTHATTHISSYSTEMCALLBASICALLYREALIZETHEAMPLIFITIONOFDNASAMPLES’KEYWORD|CFPCR；FPGA；TEMPERATURECONTROL；ELECTROPHORESISDETECTIONIIL‘一

简介：丰富多彩的自然界为人类的仿生机械提供了良好的样本。仿生学自上世纪60年代诞生以来，人们通过学习、模仿、复制和再造生物系统的结构，功能，原理及控制机制，已经开发或改进了各种各样的仿生机械，仪器，建筑和工艺。近年来，随着信息、医疗、航空、航天、军事、公安等科技的迅速发展，对直线型、特别是细长型驱动装置的需求明显增加。超细直线微电机与微驱动器可以突破现有电机的应用局限，将在工业、农业、医学、军事以及航空航天事业中扮演越来越重要的角色。本文主要借鉴了鳗鱼、水蛇、精子等细长型水生动物和微生物的运动机制，模仿它们在液体中的泳动姿态，致力于研究这种波动致动机理在超细直线微驱动器中的可行性。本论文得到国家自然科学基金项目“精子泳动机制及其在微机电系统驱动中的应用研究”编号50377022的资助。本文的主要工作如下首先，研究了鱼类波动推进机制，在LIGHTHILL的细长体理论基础上，推导了一般和特殊波动函数作用下的推进力和推进效率公式，并分析了各种参数对于推进力和推进效率的影响。本文首次采用有限元的方法对细长体波动运动进行流固耦合分析，从结构和流场的交互影响中分析了体形参数和运动参数对于波动的影响，得出了与理论分析相一致的结论，分析的结果将对未来的泳动微机械装置的优化设计提供了参考。最后，本文还设计了一种新型的可在水中自主游动的泳动微机械装置，从实验的角度对模拟的结果进行了验证。实验结果表明，改变输入电压将改变微机械的波动频率，进而改变其前进速度，结果也同时表明了流固耦合分析的准确性。

简介：上海交通大学硕士学位论文生物机电一体化假肢手的仿真系统姓名宋瑞岭申请学位级别硕士专业机械电子工程指导教师朱向阳20080201上海交通大学硕士学位论文IVSIMULATIONFBIOMECHATRONICPROSTHETICHABSTRACTTHEUNDERACTUATEDMECHANISMSAREWIDELYUSEDINTHEDESIGNDEVELOPMENTOFPROSTHETICHSINTHISTHESISASIMULATIONSYSTEMFUNDERACTUATEDEMGCONTROLLEDPROSTHETICHSISINTRODUCEDTHESIMULATIONSYSTEMCANBEUSEDFCOMPUTERAIDEDANALYSISOFTHEKINEMATICSTHEFCETQUEDISTRIBUTIONPROPERTIESFCOMPUTERAIDEDDESIGNOFTHEUNDERACTUATEDHINADDITIONTHESYSTEMPROVIDESANEXPERIMENTALENVIRONMENTFTHEDEVELOPMENTOFBIOMACHINEINTERFACEWHICHISTHEKEYFUNCTIONALMODULEOFABIOMECHATRONICHTHEPRIMARYCONTRIBUTIONSOFTHETHESISARESUMMARIZEDASFOLLOWSFIRSTTHEKINEMATICSTATICMODELSOFLINKAGETYPEUNDERACTUATEDPROSTHETICHAREDERIVEDTHEMODELSHAVEBEENUSEDFTHEIMPLEMENTATIONOFTHESIMULATIONSYSTEMTHEFACTSWHICHINFLUENCETHEFCEDISTRIBUTIONPROPERTIESOFTHEUNDERACTUATEDHISINVESTIGATEDSECONDTHEBIOSIGNALEMGACQUISITIONPROCESSINGSOFTWARESYSTEMISDEVELOPEDTHECOMMUNICATIONBETWEENTHEPROSTHETICHTHECOMPUTERISIMPLEMENTEDVIAUSBTHESYSTEMISCOMPRISEDOFTHEDSPFIRMWAREPCAPPLICATIONSOFTWARETHEFEATUREEXTRACTIONPATTERNCLASSIFICATIONALGITHMSAREALSODEVELOPEDEMBEDDEDINTHESYSTEMLASTUSINGTHESIMULATIONSYSTEMTHESIGNALACQUISITIONPROCESSINGSYSTEMANONLINEEXPERIMENTALSETUPISDEVELOPEDTHEEXPERIMENTALSETUPCANSIMULATETHEPROCESSHOWTHEHUMANBEINGSCONTROLTHEMANIPULATIONOFPROSTHETICHSVIAEMGSIGNALSTHEBIOMACHINEINTERFACEISCAPABLEOFCOLLECTINGTHEEMGSIGNALSFROMTHEFEARMDECODETHEMOTIONATTENTIONOFTHEHUMANBEINGSGENERATETHEMOTIONCOMMTOCONTROLTHEMANIPULATIONOFTHEVIRTUALPROSTHETICHTHESIMULATIONSYSTEMPROVIDESACOMPUTERAIDEDTOOLFTHEDEVELOPMENTOFUNDERACTUATEDPROSTHETICHASWELLASTHEBIOMACHINEINTERFACEKEYWDSPROSTHETICHSIMULATIONSTATICSKINEMATICSELECTROMYOGRAPHICSIGNALS

简介：天津大学硕士学位论文生物电阻抗成像数字化系统设计姓名曲志刚申请学位级别硕士专业检测技术与自动化装置指导教师王化祥20050101ABSTRACTCHARACTERIZEDBYTHELOWCOST，SMALLVOLUME，NONINVASIVENESSTOHUMANBODIES，CONTINUOUSMONITORINGANDOTHERADVANTAGES，THETECHNOLOGYOFBIOLOGICALELECTRICALIMPEDANCETOMOGRAPHYHASBECOMEAVERYPROSPEROUSNONDESTRUCTIVEDETECTINGTECHNOLOGYEVERSINCEITSINVENTIONANALOGUEFILTERWHICHISWIDELYADOPTEDINTHETECHNOLOGYOFBIOLOGICALELECTRICALIMPEDANCETOMOGRAPHYHASBEENTHEBOTTLENECKOFIMAGINGSPEEDMOREOVERITSCAPACITYTOENHANCETHESPEEDISLIMITEDDUETOTHEPERFORMANCESOFCUTOFF行EQUENCYTHEDEGREEOFTHEPRECISIONIMPROVEMENTISLIMITEDINTHEANALOGUEFILTERANDANALOGUEDEMODULATION，WHICHAREINTERFEREDWITHTHELIMITATIONOFTHEINTRINSICPRECISIONANDPERFORMANCESOFTHECARDOFCIRCUITBOARDASWELLASMANY6THERFACTORSAIMEDATSOLVINGTHEABOVEMENTIONEDPROBLEMSINTHEBIOLOGICALELECTRICIMPEDANCETOMOGRAPHYTHEAUTHORBRINGSFORWARDTHESTUDYONTHEDIGITALIZEDSYSTEMOFTHEBIOLOGICALELECTRICIMPEDANCETOMOGRAPHYTEELMOLOGYTHEMAINRESEARCHWORKANDACHIEVEMENTSAREPRESENTEDASFOLLOWS1THEDIGITALIZEDHARDWARESYSTEMOFBIOLOGICALELECTRICIMPEDANCETOMOGRAPHYTECHNOLOGYISDESIGNEDANDACHIEVEDONTHEBASISOFTHEFORMERSYSTEMWHICHHEREINTOHASBEENINNOVATED2THEDESIGNEDSYSTEMISBASEDONTHECOILABORATIONOFDSPANDFPGATOPROVIDESPACEFORTHEFUTUREUPGRADINGOFHARDWARES，3ITDESIGNSTHEDIGITALTILTING，FIFOANDDIGITALDEMODULATINGALGORITHMONTHEBASISOFTHECYCLONECHIP，ANDTHECORRESPONDINGALGORITHMCOMPILAFIONANDEMULATIONAREALSOCONDUCTED4ITFURTHERSTHESTUDYONALGORITHMOFDIGITALTILTINGCOMPARESTHENOWADAYSPREVALENTFULLYPARALLELALGORITHMANDDISTRIBUTEDALGORITHM；ANDMAKESELABORATEEMULATIONBASEDONTESTBENCH，WHICHISCOMPILEDWITHMANYMETHODSTHERESULTOFEMULATIONPROVESTHATTHEALGORITHMHASBEENOFGOODEFFECTFINALLYSOMESUGGESTIONSTOFUTTHARIMPROVETHESYSTEMAREPRESENTEDBYTHEAUTHORKEYWORDSBIOLOGICALELECTRICALIMPEDANCETOMOGRAPHYDIGITALIZED，FPGA，DSPDIGITALTILTING，DIGITALDEMODULATION，PGA

简介：浙江大学硕士学位论文叶绿素A激光光致发光的光电检测系统及其应用研究姓名宋奕申请学位级别硕士专业测试计量技术及仪器指导教师张冬仙章海军20070501浙江大学硕士学位论文A含量和其相对应的荧光强度比F685／F735拟合出线性回归方程，再将未知叶绿素A含量的待测叶片所对应的荧光强度比F685／F735代入回归方程，即可计算得出叶绿素A的实际含量G／CM2实验表明该方法是完全可行的，且此方法相对于传统的单一分光光度法具有灵敏度高、误差小、快速简便、检测周期短等优点，相比于现有的叶绿素仪更加适用于需要定量计算叶绿素A含量的场合关键词叶绿素A含量；激光光致发光分光光度法；荧光分析法；荧光光谱；荧光相对强度；荧光峰值强度比F685／F735Ⅱ

简介：东南大学硕士学位论文一种多通道核酸检测仪的研制姓名施华南申请学位级别硕士专业生物医学工程指导教师朱纪军20050601东南人学坝．1J学位论文ABSTRACTNOWDAYS，SANGERMETHODISCOMMONLYUSEDFORSEQUENCEDETECTIONOFNUCLEICACID，THEADVANTAGESOFTHISMETHODMAINLYLIEINITSSTRONGABILITIESFORUNCHARTEDDNASEQUENCEANALYSJS，LONGSEQUENCEDETECTJONBYUSINGUNIDIRECTIONREACTION，ETCANDOVER1OOOBPCANBEACHIEVEDWHENSUCHATECHNOLOGYJSUTⅢZED．HOWEVER，THEDEVICESFORAUTOMATICDNASEQUENCETESTBASEDONTHESANGERMETHODAREUSUALLYTOOEXPENSIVE，ANDCOMPLICATED．FURTHERMORE，SEQUENCEVALIDATIONSAREMOSTLYCARRIEDONFORKNOWNDNASEQUENCESFORWHJCH100BPORBELOWISENOUGH．ASARESULT，SANGERISNOTTHEMOSTSUITABLEMETHODFORSHORTSEQUENCEANALYSIS．RECENTLY，THEDNASEQUENCEANALYSISTECHNOLOGYBASEDONTHENEWDEVELOPEDPYROSEQUENCINGTECHNO】OGYISPROVENTOBETHEEXACTMETHODFORMOSTSUCHAPPLICATIONS．INTHISDISSENATION，ANAUTOMATICDNASEQUENCEDETECTIONAPPARATUSBASEDONTHEPYROSEQUENCINGTECHNOLOGYISDEVELOPED．THISINSTRUMENTASSUMESX一86PCASUPPE卜COMPUTER，AT89C51ASDOWNCOMPUTER，PHOTOMULTIPLIERPMTASPHOTOELECTRICCONVERSIONDEVICE，HY一6022ASDATAC011ECTIONDEVICE，VISUALC6．0ANDKEILCASPROGRAMMETOOLS．THECONTROLSYSTEMISDESIGNEDBASEDONWINDOWSPLATFORM．INTHEREACTIONPROCESSOFNUCLEICACIDWITHOTHERREAGENTS，REAL一TIMEDATAC01LECTION，ANALYSISANDPROCESSARECARRIEDONFORFLUORESCENCESIGNALSENGENDEREDINTHETUBESTOREALIZETHEMULTICHANNELDETECTIONFBRNUCLEICACIDSEQUENCES．DETA订EDSOFTWAREANDHARDWAREDESIGNSFORTHEWHOLESYSTEMAREPRESENTED．FINALLY，WEVALIDATETHEFUNCTIONOFTHEINSTRUMENTTHROUGHEXPERIMENTS．KEYWORDSPYROSEQUENCING，UPPE卜COMPUTERDOWNCOMPUTERPHOTOMULTIPLIER，MULTICHANNELDETECTION，ⅥSUALC6．O，KEILC

简介：随着现代科学技术的发展，对工程机械结构提出了越来越高的要求。一方面要求工程机械本身具有较高的承载能力、高的疲劳寿命，另一方面还要求工程机械在满足刚度和强度的前提下，求得最优设计，提高工作效率，降低生产成本。装载机广泛应用于水电、矿山、交通和建筑等部门，其触土部件－铲斗的结构、性能直接影响整台设备的工作效率。当装载机以粘湿物料为工作对象时，工作部件－铲斗存在严重的物料粘附问题，这不仅要降低装载机的工作效率，还要增加工作时的阻力，因此，要求人们对其进行减粘降阻的研究。生物经过长期与自然环境进行物质、能量及信息的交换，造就了适应生态环境的生物系统和生存本领。某些动物的爪趾经过长期进化，逐步形成了具有特定曲率的弯曲形状，表现出优良的减阻功能，这为改进触土工作部件触土面的几何形状，乃至优化其在工作中的力学性能提供了仿生研究的基础。本文在某厂生产的装载机铲斗的基础上，根据田鼠爪趾内轮廓线设计了两种仿生装载机铲斗。分别建立了其和土壤相互作用的二维和三维的有限元模型，并用非线性有限元法对比分析了所设计的仿生装载机铲斗和某厂原型铲斗的切削性能，分析结果表明所设计的仿生模型性能优于某厂原型铲斗。

简介：小分子荧光探针具有响应快、灵敏度和选择性高的优点，在分析检测和生物成像等方面具有重要的应用。其设计合成已成了热门的研究领域。铜离子和H2S分子均为环境中和生物体内重要的物质。目前响应的荧光探针常存在水溶性以及特异性差等问题。本论文针对该问题，设计合成了两种新型的荧光探针分子，具体内容如下1引入由纯天然氨基酸组成的三肽GLYGLYHISGGH为铜离子识别基团，该三肽具有水溶性好，对铜离子特异性强。以氨基酸TRP为荧光信号基团，采用固相合成法得到了铜离子探针TRPGLYGLYHISWGGH。由于铜离子的荧光淬灭效应，在探针WGGH中识别基团与铜离子结合后会导致TRP的荧光发生淬灭。从而能实现对铜离子的ONOFF型响应。该探针具有响应速度快、水溶性好、检测窗口宽、检测限低以及选择性好等特点。其荧光强度与铜离子浓度的对数在101000NM范围内呈良好的线性关系，检测限为35NM。2在工作1基础上，将具有较长发射波长的香豆素荧光骨架与铜离子识别基团GGH通过固相法偶联，合成了荧光探针分子CGGH。该探针分子同样具有响应速度快、水溶性好以及高选择性等特点。其对铜离子的响应平衡时间小于一分钟，检测范围为120ΜM，检测限为028ΜM。进一步，以CGGHCU2复合物为探针分子，利用S2对CU2的强亲和性，实现了对H2S的检测，其对H2S的检测范围为420ΜM，检测限为047ΜM。由于探针CGGHCU2具有良好的水溶性和细胞膜透过性，利用探针CGGHCU2成功实现了对HELA细胞的H2S的荧光成像。

简介：重庆大学硕士学位论文原子力显微镜对聚丙烯酰胺凝胶以及细胞结构和力学性质的研究姓名范霞申请学位级别硕士专业生物物理学指导教师叶志义20090605ABSTRACTMOSTOFCELLPHYSIOLOGICALANDPATHOLOGICALPROCESSES，SUCHASDEVELOPMENT，DIFFERENTIATION，AGINGANDCANCERIZATION，AREALWAYSACCOMPANIEDBYCYTOSKELETONRECONSTRUCTIONANDCHANGESINCELLULARMECHANICALPROPERTIESCONSTRUCTINGASYSTEMTOSIMULATEINTERNALENVIRONMENTINVITROANDINVESTIGATINGCELLMECHANICALPROPERTIESWITHITISMEANINGFULTODISEASESDIAGNOSISANDTHERAPYANDDRUGSCREENINGTHEATOMICFORCEMICROSCOPYAFMHASBEENANEXTREMELYIMPORTANTTOOLFORBIOLOGICALMECHAMICSDUETOITSABILITYTOMEASURESAMPLEPROPERTIESWITHSUBNANOMETERRESOLUTIONINAIRORINLIQUIDCONDITIONSANDWITHMINIALDESTRUCTIONITCANNOTONLYPROVIDESTHECAPABILITYTESOLVESAMPLEFEATURESATNANOMETERSCALEBUTITALSOPROVIDESLOCALMECHANICALINFORMATIONOFSURFACEINTHISTHESISITFOCUSEDONTHESTUDYOFTHESTRUCTUREANDMECHANICALPROPERTIESFORTHELUNGCANERCELLA549ANDPOLYACRYLAMIDEGELWITHAFMTHEPURPOSEOFTHISWORKISTOSETUPAPRACTICALWAYFORDETERMININGTHEMECHANICALPROPERTIES，ANDMAKINGATHEORYBASISFORTHEFUTUREWOKTHEFOLLOWINGARETHEMAINCONTENTSANDRESLUTSOFTHEPRESENTSTUDY1THEIMAGEOFTHEPOLYACRYLAMIDEGELCANBEOBTAINEDWITHTHEBENYUANCSPM5000FROMTHEPICTUREWECANSEETHATTHEAVERAGEROUGHNESSISNOMORETHAN05NMWHENTHEPAGELSAREPLOYMERIZEDWELLANDHAVEAGOODSPREADINGONTHECONTRARYITWOULDFORMEDLOTSOFSMALLHOLESWHOSEDEPTHISVARIEDFROM3NANOMETERTO5NANOMETERMOREOVERTHEYOUNG’SMODULUSCANBEOBTAINEDBYFITTINGTHEAPPROACHINGCURVEWITHHERTZEMODELANDTHEVALUEISABOUT28KPAAT75％ACRYLAMIDEAND06％BISACRYLAMIDE2AFTERIMAGINGWITHAFM，WECANFINDTHATTHELUNGCANCERCELLSHAVEASPINDLYSHAPETHELENGTHFROMTHELONGAXISDIRECTIONISABOUT4060NMANDTHETHICKNESSOFTHECELLISUPTO3“MFURTHERMORE，BESEDONHERTZIANFITOFTHEFORCECURVES，THEAPPARENTELASTICMODULUSOFTHECELLSWASFOUNDTOBEAFUNCTIONOFTHELOADINGRATEFORINCREASEDLOADINGRATES，THELIVINGCELLSWEREFOUNDTOEXHIBITANINCREASECHANGEOFSTIFFNESS，WHEREASFORTHEFIXEDCELLTHEREWASLITTLESTIFFENINGATEQUIVALENTLOADINGRATE，ANDITKEPTITSELASTICMODULUSATTHEVALUEOF20KPAITISINTERESTINGTOFINDTHATTHEREWERELOTSOFRUPTUREENVENTSFORTHEFIXEDCELLANDTHEMEANRUPTUREFORCEWOULDINCREASEWITHTHEDECREASEOFTHELOADINGRATE，BUTITCANNOTBESEENFORLIVINGCELL1I

简介：工学硕士学位论文荧光法海藻浓度在线测量系统的研究李冰燕山大学2010年5月CLASSIFIEDINDEXTN29UDC62138DISSERTATIONFORTHEMASTERDEGREEINENGINEERINGTHEREASERCHONTHEONLINESYSTEMTOMEASUREALGAECONSISITENCEBYFLUORESCENCECANDIDATELIBINGSUPERVISORPROFBIWEIHONGACADEMICDEGREEAPPLIEDFORMASTEROFENGINEERINGSPECIALITYPHYSICALELECTRONICSUNIVERSITYYANSHANUNIVERSITY

简介：华南师范大学硕士学位论文相关谱技术在生物大分子检测上的应用姓名李军锋申请学位级别硕士专业光学指导教师邢达20050601摘要摘要相关谱技术是一种统计方法，它通过对时间序列的涨落信号作相关运算自相关和互相关，从而得到引起涨落过程的参数，例如扩散系数，浓度，结合和分离常数等。目前应用广泛的两种相关谱技术包括光子散射相关谱技术PHOTONSCATTERINGCORRELATIONSPECTROSCOPYPSCS和荧光相关谱技术FLUORESCENCECORRELATIONSPECTROSCOPYFCS。参与改进了本实验室自行研制的小型低强度激光光子散射相关谱仪。与现有的同类型仪器相比，该仪器采用双层折射率样品匹配池，从而可以减小杂散光的干扰；采用带梯度折射率透镜的单模光纤接收、传输散射光信号提高了散射光的收集传输效率；用低强度的激光避免了对样品的辐射损伤，同时可以使激光器和系统集成在一起，整个系统小型实用。用标准的不同粒径的聚苯乙烯球检验了自行设计的系统，系统的测量范围从几个纳米到一个微米都很精确。利用光子散射相关谱技术研究了牛血清白蛋白分子的扩散系数在不同强度离子溶液中随着蛋白分子浓度变化的规律。结果表明在低离子强度环境中蛋白的扩散系数随蛋白的浓度增加而增加，随着离子强度的逐步增大，蛋自分子的扩散系数随其浓度的增加而减小。利用平均势场理论的两体硬球相互作用模型解释了蛋白分子的相互作用变化规律随着离子强度的增加，离子氛厚度减小，蛋白分子间的相互作用由排斥变为吸引，蛋白开始聚集。根据扩散系数随蛋白浓度变化的斜率与离子浓度变化的相关性，回归出了蛋白相互作用参数，蛋白所带的有效电荷ZP一90E、HAMAKER常量28KBT。这项工作说明了光子散射相关谱技术是研究蛋白分子相互作用的一种有效技术。参与了从德国ZEISS公司进口的荧光相关谱仪的组装工作。与现有的同类型仪器相比，该仪器采用双通道探测，不仅可以同时进行自相关荧光谱测量，还可以进行荧光互相关光谱测量；该仪器装有三个激光器光源，一个氩离子激光器和两个氦氖激光器，可以提供五种波长的激光，从而扩大了染料分子的选择余地；该仪器测量时间短，灵敏度高，平均10秒钟1个样品，可以检测到单个荧光分子该仪器完全由计算机控制，操作简单。

简介：华中科技大学硕士学位论文光学相干层析成像系统的研制与实验研究姓名陈筱勇申请学位级别硕士专业生物医学工程指导教师杨玉星20040601VABSTRACTBYCOMBININGSOMEADVANTAGESOFOPTICALHETERODYNEDETECTIONSCANNINGTOMOGRAPHYOPTICALCOHERENCETOMOGRAPHYOCTPROVIDESIMAGESWITHHIGHSENSITIVITYHIGHRESOLUTIONINTRANSVERSEDEPTHDIMENSIONSITISANOVELTECHNIQUEIMAGINGFROMTHESCATTERINGMEDIUMSUCHASTHECREATURETISSUEITSNONINVASIVEACTERISTICEASEOFUSEINVIVOPROMOTEDITSRAPIDLYDEVELOPMENTINRECENTYEARSSINCEITWASBROUGHTFWARDSEVERALYEARSAGOFIBERSOMERELATEDPRODUCESGETMATUREWITHDEVELOPMENTOFTELECOMAPPLIEDFIBERPRODUCESTOOCTTHEWHOLESYSTEMCHANGESVERYCOMPACTITSLOWCOSTMAKESITSWIDEAPPLICATIONPOSSIBLEINCLINICALMEDICINEINTHISTHESISWESTUDIEDTHESYSTEMDESIGNAPPLICATIONBASEDONOCTIMAGINGPRINCIPLEMETHODANALYZEDTHEOCTPRINCIPLETHEPROBLEMSINPREVIOUSOCTSYSTEMOFOURLABATYTHISTHESISPRESENTEDAPLANTOREBUILDTHISSYSTEMTHEMODIFIEDOCTSYSTEMCOULDIMAGETHETISSUEAUTOMATICALLYITHASTHEADVANTAGESOFHIGHSENSITIVITYHIGHCAPABILITYPRICERATETHEMODIFIEDSYSTEMISALOWCOSTOCTSYSTEMWITHOPTICSMECHANICSELECTRICSPARTSTHESYSTEMUSEDTHESLDLIGHTSOURCEWITHWAVELENGTHOF1310NMHALFWIDTHOF30NMSOTHESYSTEMHASTHERESOLUTIONOF25MINLONGITUDE24MINLATITUDETHEHARDWAREWASBUILTWITHDOUBLECPUSTHEPERSONALCOMPUTERCONTROLLEDTHEWHOLESYSTEMCONVENIENTLYDISPLAYEDTHERESULTINREALTIMETHELONGITUDESCANNERBASEDONMICROCOMPUTERREDUCEDTHERELATIVEERROFSCANNINGSPEEDTOONEPERCENTSOTHESCANNINGSYSTEMWASDIGITIZEDTHESTABILITYINTELLIGENCEOFTHESYSTEMISADVANCEDTHENTHEOCTSYSTEMCOULDBEUSEDINREALTIMEIMAGINGOFTISSUESAMPLEHIGHQUALITYIMAGESOFSTARDTISSUESAMPLESOBTAINEDWITHTHEMODIFIED

简介：浙江大学理学院物理系博士学位论文生物大分子构象的理论与模拟姓名姜舟婷申请学位级别博士专业凝聚态物理指导教师章林溪叶高翔20050101浙江大学博士学位论文Y0的长程相关性和自相关性。同时，通过对能谱分析发现，编码DNA在33310一BP’1处有一个明显的峰值，而非编码DNA没有这个峰值，这为区分DNA提供一条新途径。最后，通过对DNA这类刚性高分子链动力学行为的研究，得到弛豫时间和持久长度之间的关系，在柔性链极限下，弛豫时间满足ROUSE模型‘OCL2N2；在刚性链极限下，弛豫时间OOCL／2N1】4。这些研究结果涉及DNA的一级结构序列、三维结构空间构象和动力学行为，系统的研究为进一步了解DNA的结构和生物功能提供了理论依据。在本文第四章中，我们采用实验方法对不同离子源的结冷胶在玻璃基底表面的摩擦行为进行研究。结冷胶是多糖凝胶的一种，在工业上具有广泛的用途。在我们的工作中发现，不管是NA结冷胶还是CA2结冷胶都具有非常低的摩擦系数，特别是在运动速度较小的情况下，这使它具有动物关节替代品的独特优势。由不同离子源形成的结冷凝胶的摩擦特性并不相同，原因在于其不同的交联方式。此外还发现结冷胶在水溶液中并不稳定，一定的时间范围内，摩擦系数会随着浸润时间的增加而增加，这是由于形成结冷凝胶的网络结构不稳定造成的。这些研究揭示了凝胶的摩擦机理，同时为开拓结冷胶新的应用领域提供了理论支持。关鳝爆蛋白质，DNA，多糖凝胶，构象，疏水性，MONTECARLO模拟，弹性竿模型，摩擦行为2